FastQCFastQC Report
Sat 4 Mar 2017
G94-M6_GTTTCG_seqtk.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG94-M6_GTTTCG_seqtk.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15241632
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22100.014.4361881
TATGCCG24950.012.6969363
CGACGAT13650.011.6058431
ATGCCGT29200.011.5266024
CTCGAAT20150.010.9194751
CGTTTCG9250.010.2275333
CCGTCTT36500.09.3415157
CCGGCAT19950.09.0437341
CCGGAAT16700.08.95921
GCCGTCT37700.08.9274746
CGCCACT22000.08.8010971
CCGCAAT8100.08.6924411
GTATGCC37350.08.5994212
CGAAGAT22350.08.5648251
TGCCGTC39450.08.5317875
CCCGGTT15500.08.3752371
CGCAATT7750.08.2332841
TCGTATC10200.08.1957822
CGCCAAT11750.08.0520671
CGACAAT9350.08.0009971
Produced by FastQC (version 0.11.2)