FastQCFastQC Report
Fri 25 Nov 2016
G94-M6_GTTTCG.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG94-M6_GTTTCG.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15241632
Sequences flagged as poor quality0
Sequence length67
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32000.013.8221291
CGACGAT17500.012.5502211
TATGCCG35500.012.37146953
CTCGAAT27350.011.1532031
ATGCCGT42000.011.1100244
CGTTTCG14100.09.3019433
CCGCAAT10750.09.0802631
CGCCACT29700.09.0382251
CCGTCTT52500.09.0039077
CGCAATT9900.08.9355191
CCGGCAT28050.08.9173941
GCCGTCT52600.08.87083156
CCGGAAT23500.08.8266931
CGAAGAT30850.08.6024281
CGCGAAT5703.2717253E-78.562531
CCGGGAT36450.08.5361011
CGACAAT12400.08.3640031
CCCGGTT21550.08.3514461
CGTCGAT6604.04998E-88.3192761
TGCCGTC56550.08.2515325
Produced by FastQC (version 0.11.2)