FastQCFastQC Report
Fri 25 Nov 2016
G94-M3_GATCAG.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG94-M3_GATCAG.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10192335
Sequences flagged as poor quality0
Sequence length67
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20350.016.0386961
TATGCCG20850.015.6517723
ATGCCGT28700.011.689234
CGCGTTT3051.3604093E-511.0012631
CCGGAAT15450.010.6614181
CCGAAAT9350.010.4397011
CTCCGAT17000.010.4070761
CCGTCTT34850.09.9761837
CTCGAAT15600.09.9722981
GCCGTCT34800.09.8152446
CCGGGAT21200.09.4963721
CGACAAT8050.09.4731371
CAATCGA5208.035022E-89.3840814
CGCAAAT8550.09.2759181
TGCCGTC36950.09.244495
GTATGCC37000.09.2322242
CGCCTAT6655.14774E-109.17398551
CCCGCAT14300.09.1723811
CGACGAT9350.09.1347391
CGGGGAT20100.08.9537641
Produced by FastQC (version 0.11.2)