FastQCFastQC Report
Fri 25 Nov 2016
G146-M30_GCCAAT_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M30_GCCAAT_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17575309
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA24600.026.9265841
TCGTATG26000.024.7083943
CGCCAAT26900.023.8077933
CGTATGC27150.023.2547944
ATCGGAA28450.023.1917922
GTCACGC27900.023.178929
ACACGTC29100.022.9003713
ACGCCAA28150.022.82469232
TATCTCG26650.022.2992739
AGTCACG30500.021.34607928
CTCGTAT28850.021.2779842
ACGTCTG31650.021.12420515
CGTCTGA31900.021.02771616
TCACGCC34650.019.29834430
TCTCGTA34150.017.3986541
CGGAAGA40850.017.3364094
CACGTCT39250.017.25853314
CACACGT38950.017.16559812
CACGCCA40300.016.37446631
TCGGAAG49800.014.2648923
Produced by FastQC (version 0.11.2)