FastQCFastQC Report
Fri 25 Nov 2016
G146-M29_ACAGTG_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M29_ACAGTG_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20921679
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT18050.022.67370611
CGTCGTG21150.018.83033812
TCGTGTA22800.017.27315714
GATCTCG25000.016.36603234
GATCGGA27750.015.5440431
AGCGTCG27050.015.2114810
CGTGTAG27300.015.06976815
GTCGTGT27450.014.66900613
GTAGGGA30000.013.85939518
GTAGATC30400.013.82103731
TCTCGGT29350.013.79037436
GTCGCCG27400.013.56803644
GAGCGTC32150.013.2776139
AGAGCGT33250.013.1031258
ATCGGAA35400.012.4959692
ATCTCGG33250.012.17303235
TGGTCGC31300.011.94710342
TGTAGGG36550.011.91765717
GTGTAGG37400.011.7060716
GGTCGCC33200.011.46217643
Produced by FastQC (version 0.11.2)