FastQCFastQC Report
Fri 25 Nov 2016
G146-M29_ACAGTG_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M29_ACAGTG_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20921679
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA24600.023.6092971
CGTATGC25500.021.73994344
TCGTATG27150.020.82481243
ATCGGAA28850.020.0491942
ACACGTC30850.019.6050813
CTCGTAT30000.017.89535742
CGTCTGA33750.017.7242816
ACGTCTG35450.017.37049315
CACGTCT41300.015.23025314
TCTCGTA35150.014.08641941
CACACGT44850.013.77954912
CGGAAGA46050.013.4203444
GCACACG50500.012.49918411
GATCTCG42750.011.73895539
TCGGAAG53900.011.5474123
ATCTCGT43450.011.39696340
AGTGATC48750.010.24795636
GTCGGTT22750.09.8039791
TCGGTTT28600.09.6123812
CTCCGAT26700.09.2633721
Produced by FastQC (version 0.11.2)