FastQCFastQC Report
Fri 25 Nov 2016
G146-M28_TGACCA_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M28_TGACCA_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17398747
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT17750.024.66851211
CGTCGTG19950.022.05846612
GATCGGA24150.021.1408041
TCGTGTA21800.019.88184714
GATCTCG24750.018.30908634
CGTGTAG24400.018.21340415
GTCGTGT24500.018.05170413
TCTCGGT25150.017.40517836
GAGCGTC27950.016.2963569
AGAGCGT29000.015.933998
AGCGTCG28050.015.84589310
GTAGGGA29200.015.74609918
GTAGATC29150.015.62145231
GTCGCCG26200.015.53258744
ATCTCGG30500.014.78529235
ATCGGAA34900.014.439912
GGTCGCC31250.013.79631743
GTGGTCG29500.013.7194941
TGGTCGC32100.013.4995242
GTGTAGG34800.013.46588516
Produced by FastQC (version 0.11.2)