FastQCFastQC Report
Fri 25 Nov 2016
G146-M22_GAGTGG_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M22_GAGTGG_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17897379
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG258500.14443455659066057Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210050.11736355362424856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT43450.036.9182511
CGTCGTG47350.034.3886512
GATCGGA54750.033.4861371
CGTGTAG49650.032.9675515
TCGTGTA49700.031.91776714
AGCGTCG52550.031.1541410
TCTCGGT51700.031.01594536
GATCTCG53400.030.72931334
GAGCGTC54050.030.289639
GTCGTGT53750.030.08939613
GTAGATC55350.029.88640831
GTAGGGA55450.029.75441618
GTCGCCG53950.028.9490544
AGAGCGT58000.028.6823148
TGGTCGC54550.027.94394542
GTGGTCG55200.027.93258741
GGTCGCC56250.027.76411443
TGTAGGG60600.027.62617317
ATCTCGG59400.027.55112835
ATCGGAA66450.027.325492
Produced by FastQC (version 0.11.2)