FastQCFastQC Report
Fri 25 Nov 2016
G146-M22_GAGTGG_AC9EP5ANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M22_GAGTGG_AC9EP5ANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17897379
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT407670.22778195622945685TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA62200.036.870871
ACGAGTG62450.036.11534532
CTCGTAT61850.034.14579844
CGAGTGG66000.034.1121133
TCACGAG66800.034.0471430
CACGAGT66650.033.95875531
ATCGGAA67400.033.936062
ACACGTC68500.033.87204413
TATCTCG59500.032.84343341
CGTCTGA70900.032.69343616
ACGTCTG72850.031.99944715
TCTCGTA62550.031.55039843
GTCACGA73850.030.94579129
AGTCACG74850.030.82509628
CACACGT76550.030.65481812
CACGTCT78000.029.85938514
GCACACG84100.028.03356411
CGGAAGA82750.027.9067544
ATCTCGT70850.027.79538342
TCGGAAG85300.026.8662283
Produced by FastQC (version 0.11.2)