FastQCFastQC Report
Fri 25 Nov 2016
G146-M17_GTCCGC_AC9EP5ANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG146-M17_GTCCGC_AC9EP5ANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16394331
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG865130.5277007033711837Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA284620.17360879196595458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT127250.041.94827311
CGTCGTG130350.041.18710312
GATCGGA152850.040.729671
TCGTGTA127950.040.70182814
GTCGTGT130800.040.55818613
AGCGTCG133100.040.4856510
CGTGTAG134500.040.01027715
GTCGCCG123850.039.92804344
TCTCGGT132800.039.91922836
GATCTCG134250.039.8490334
GAGCGTC137500.039.4302679
ATCTCGG136000.039.40132535
ATCGGAA154850.039.195342
GTGGTCG127450.039.0213941
TGGTCGC125250.038.91794642
AGAGCGT141250.038.5861938
GGTCGCC128450.038.5305343
AAGAGCG149200.037.709987
CGGTGGT135400.037.70277439
TCGGTGG144500.037.47528538
Produced by FastQC (version 0.11.2)