FastQCFastQC Report
Fri 25 Nov 2016
G145-M9_GATCAG_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M9_GATCAG_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18909032
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2807351.48466087528965Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA484590.2562743560854939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT374700.042.21098311
CGTCGTG376450.042.14852512
GATCTCG377500.041.62919634
TCGTGTA376650.041.4941214
GATCGGA407650.041.489171
CGTGTAG383250.041.38039815
AGCGTCG384950.041.22754310
GTCGCCG359000.041.21874644
ATCTCGG382600.041.20423535
TCTCGGT378650.041.1976236
GTAGGGA384400.041.08365618
GTAGATC390650.040.9278631
GAGCGTC388950.040.7714469
ATCGGAA411550.040.721722
AGAGCGT393050.040.6819348
GTCGTGT388700.040.65506713
GGTCGCC362100.040.6458843
GTGGTCG367150.040.6102741
TGGTCGC361100.040.44445442
TGTAGGG398900.040.21979517
Produced by FastQC (version 0.11.2)