FastQCFastQC Report
Fri 25 Nov 2016
G145-M8_ACTTGA_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M8_ACTTGA_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17798055
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG564670.3172650045187522Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183140.10289888417582707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT77100.040.52707711
CGTCGTG80650.039.01492312
CGTGTAG83000.037.80137315
AGCGTCG84150.037.4437610
TCGTGTA83850.036.94751714
GATCGGA91550.036.943381
GATCTCG86050.036.63308734
GTCGTGT86150.036.3958113
GAGCGTC87850.036.118019
GTCGCCG80350.035.91734344
ATCTCGG87600.035.8575335
TCTCGGT88750.035.0198436
GTAGATC91400.034.9713431
GTAGGGA90600.034.76916518
GTGGTCG85850.033.89365841
TGTAGGG96000.033.36428517
AGAGCGT96950.033.2499778
ATCGGAA100800.033.051142
GTGTAGG97650.032.66875516
GGTCGCC89400.032.4521943
Produced by FastQC (version 0.11.2)