FastQCFastQC Report
Fri 25 Nov 2016
G145-M8_ACTTGA_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M8_ACTTGA_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17798055
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC747890.42020883742633675TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA95500.039.4330141
TCGTATG99700.037.75658843
ATCGGAA100300.037.605572
ACACGTC105350.036.6582413
ACGTCTG105850.036.5920515
CGTATGC102700.036.58939744
CTCGTAT98950.036.35275742
AATCTCG93950.036.3105439
CGTCTGA110550.034.91697716
CACGTCT113150.034.15284714
TCTCGTA101000.034.07263641
CACACGT114800.033.56401412
CGGAAGA118300.032.329864
GCACACG119900.032.22715811
ATCTCGT108650.031.74104140
TTGAATC113500.030.40204436
TCGGAAG128500.029.6609043
GAATCTC126850.027.20460338
GTCTGAA148950.026.21069717
TGAATCT132450.026.1855537
Produced by FastQC (version 0.11.2)