FastQCFastQC Report
Fri 25 Nov 2016
G145-M7_CAGATC_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M7_CAGATC_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19643562
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG371110.18892194806624177Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT56300.037.87373711
CGTCGTG58500.036.2224412
AGCGTCG62300.034.3660710
CGTGTAG63000.034.29463615
TCGTGTA63250.033.8488614
GTCGTGT64550.033.40595213
GATCGGA70400.032.5735741
GATCTCG66250.032.2401334
GAGCGTC69850.030.7800799
GTCGCCG64350.030.4911844
ATCTCGG70900.030.34051135
TCTCGGT70200.030.17229736
GTAGGGA73000.029.55967118
GTGGTCG67450.029.18325641
GTAGATC75250.028.7066731
AGAGCGT75450.028.670328
TGGTCGC69300.028.59555842
GTGTAGG77650.028.38888716
ATCGGAA81100.027.923182
TGTAGGG79150.027.84768717
Produced by FastQC (version 0.11.2)