FastQCFastQC Report
Fri 25 Nov 2016
G145-M6_GCCAAT_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M6_GCCAAT_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17321882
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG306550.17697268691704515Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT47050.037.46051811
CGTGTAG49850.035.4856615
CGTCGTG49850.035.44449212
AGCGTCG52100.034.418510
TCGTGTA53450.032.644514
GATCGGA57900.032.3425671
GTCGTGT55450.031.9437913
GATCTCG55650.031.78134
TCTCGGT56150.031.49581536
GAGCGTC58000.030.6918229
ATCTCGG58500.030.45708335
GTCGCCG55950.029.72296544
GTAGGGA60900.029.51117318
GTAGATC61900.029.21252831
ATCGGAA65100.028.5964972
GTGGTCG60650.027.96018841
AGAGCGT64400.027.9149678
GTGTAGG65300.027.7614516
TGTAGGG65400.027.68296217
TGGTCGC62800.026.65333442
Produced by FastQC (version 0.11.2)