FastQCFastQC Report
Fri 25 Nov 2016
G145-M5_ACAGTG_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M5_ACAGTG_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19386672
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG270140.13934315286295657Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT43700.034.54316311
CGTCGTG45950.032.80331412
CGTGTAG45250.032.43243415
TCGTGTA47900.030.31833814
AGCGTCG50650.030.14912610
GATCGGA54150.029.5041681
GTCGTGT50100.029.47071813
GATCTCG50950.029.05673434
TCTCGGT55600.027.0983936
ATCTCGG56400.026.55891235
GTAGGGA56250.026.3966118
GTAGATC57400.026.21361231
GTCGCCG54400.025.999644
GAGCGTC58150.025.9587449
GTGGTCG56650.025.27452741
TGTAGGG62200.024.65020617
ATCGGAA64800.024.48532
GTGTAGG62100.024.44521916
AGAGCGT63100.024.0967248
GAGTGTA64250.023.93582327
Produced by FastQC (version 0.11.2)