FastQCFastQC Report
Fri 25 Nov 2016
G145-M4_TGACCA_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M4_TGACCA_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19728465
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG470010.2382395183811817Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT72300.038.1361111
CGTGTAG76250.036.3279815
CGTCGTG76900.036.11068312
AGCGTCG79350.035.1346510
TCGTGTA78350.034.51438514
GATCTCG81400.033.59030534
GTCGTGT82400.033.53930713
GATCGGA92600.033.295371
TCTCGGT83850.032.34337636
GTAGATC87850.032.1515231
GTAGGGA87050.031.76389318
ATCTCGG87100.031.4155135
ATCGGAA97750.031.0909212
GAGCGTC90550.030.9608089
GTCGCCG84150.030.55710244
GTGTAGG92850.030.51847616
GTGGTCG84600.030.41701941
AGAGCGT93050.030.1998488
TGTAGGG93900.030.05625517
TGGTCGC85450.029.72897142
Produced by FastQC (version 0.11.2)