FastQCFastQC Report
Fri 25 Nov 2016
G145-M4_TGACCA_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M4_TGACCA_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19728465
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC678840.3440916462583379TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA93650.037.856341
TCGTATG94750.037.31415643
CGTATGC96300.036.87348644
CTCGTAT94900.035.7486442
ATCGGAA100750.035.2114752
AATCTCG90150.034.6931539
ACGTCTG104800.034.5228115
ACACGTC106250.034.1739713
CGTCTGA106250.034.01027316
TCTCGTA98550.031.90605441
CACGTCT115050.031.71418814
CACACGT114500.031.71165512
ATCTCGT100400.031.36897740
GACCAAT109550.030.01376735
CGGAAGA119950.029.9603374
ACCAATC106950.029.42508736
TCGGAAG123300.028.8609333
ACTGACC124950.028.41740432
GCACACG129050.028.3227911
TGACCAA134300.026.54830234
Produced by FastQC (version 0.11.2)