FastQCFastQC Report
Fri 25 Nov 2016
G145-M1_ATCACG_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M1_ATCACG_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19435498
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG442530.22769161870717178Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT66650.037.17445411
CGTGTAG70700.035.1026715
CGTCGTG71000.035.02020312
TCGTGTA71600.034.35645314
AGCGTCG72700.034.26148210
GATCGGA77900.033.8990171
GTCGTGT74000.033.8671213
GATCTCG74300.033.3375834
TCTCGGT78050.031.6481136
GTAGATC80350.031.31996731
GAGCGTC80400.031.0639219
GTCGCCG76000.030.76628944
ATCGGAA84750.030.7176762
ATCTCGG82450.030.2268235
GTAGGGA82100.030.06204818
GTGTAGG86850.029.13037716
AGAGCGT87500.028.6690468
TGTAGGG87750.028.65359917
GTGGTCG83250.028.21629541
CGGTGGT85000.027.89283839
Produced by FastQC (version 0.11.2)