FastQCFastQC Report
Fri 25 Nov 2016
G145-M16_CCGTCC_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M16_CCGTCC_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18880824
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC740740.39232397908057404TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA100100.039.2096631
ACCCGTC99950.038.82705332
TCGTATG101850.037.8881443
CGTATGC104400.037.17344344
ACACGTC109300.036.50021413
ATCGGAA107950.036.366152
CGTCTGA110100.036.1973516
CTCGTAT104050.035.90344242
ACGTCTG111250.035.78363815
CACCCGT109600.035.6077431
TCACCCG113200.034.4364730
TCTCGTA103950.033.5923341
CACACGT119250.033.56544512
CACGTCT119950.033.40738714
CCCGTCC117350.033.06052833
CGGAAGA124350.031.852954
CGTCCAT113550.031.84452235
CCGTCCA123500.031.47173734
ATCTCGT112950.031.02102540
GCACACG129650.030.85534511
Produced by FastQC (version 0.11.2)