FastQCFastQC Report
Fri 25 Nov 2016
G145-M15_ATGTCA_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M15_ATGTCA_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17902178
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG187750.10487550732653869Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT32300.034.19921511
CGTCGTG35950.030.78775212
CGTGTAG36300.030.48731815
TCGTGTA37100.029.95128314
AGCGTCG37950.029.10616710
GATCTCG38450.028.31845934
GATCGGA42850.027.32161
GTCGTGT40650.027.2817113
GTCGCCG38850.025.76187744
TCTCGGT42200.025.64427436
GAGCGTC44400.024.730489
GTAGGGA45350.024.25273718
ATCTCGG45750.024.03941535
GTGGTCG42600.023.90411641
ATCGGAA49600.023.6034372
GTAGATC47300.023.5787631
TGGTCGC42550.023.41596842
GTGTAGG50400.022.5677116
AGAGCGT49250.022.4738738
TGTAGGG51200.022.38440517
Produced by FastQC (version 0.11.2)