FastQCFastQC Report
Fri 25 Nov 2016
G145-M14_AGTTCC_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M14_AGTTCC_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18589712
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG200860.10804901119500937Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT33300.033.3691911
CGTCGTG35050.032.0163112
TCGTGTA38650.028.69208114
CGTGTAG39750.028.22929415
GATCGGA43400.028.1402231
GATCTCG42200.027.78320734
AGCGTCG41900.026.93924910
GTCGTGT44500.025.56318913
TCTCGGT45250.025.51970336
GTAGGGA46400.024.69972218
GAGCGTC46800.024.4490439
GTAGATC48000.024.42679231
ATCTCGG48250.024.29833235
GTCGCCG45750.023.94424844
AGAGCGT49100.023.3933418
ATCGGAA51900.023.3195192
TGGTCGC47800.022.82365442
GGTCGCC48300.022.63389843
GTGGTCG48950.022.60138341
GTGTAGG52850.022.31285916
Produced by FastQC (version 0.11.2)