FastQCFastQC Report
Fri 25 Nov 2016
G145-M14_AGTTCC_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M14_AGTTCC_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18589712
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT254350.13682299112541388TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA40700.033.4787861
TCCGTAT40650.030.80583437
ATCGGAA46550.029.480932
CCGTATC41750.028.94232238
GTTCCGT46250.028.78981835
ACACGTC49450.028.2407813
TATCTCG43250.028.0840341
CGTCTGA50350.027.86956416
CTCGTAT46800.027.1249344
CGTATCT44450.027.08599339
AGTTCCG50100.026.79786934
TTCCGTA50650.026.67882536
ACGTCTG53050.026.6169715
CACACGT58400.024.06347812
CACGTCT60550.023.24698414
CGGAAGA62500.022.7667244
TCTCGTA53650.022.67744343
TCGGAAG65500.021.455363
GCACACG66950.021.055711
ATCTCGT61600.019.8223742
Produced by FastQC (version 0.11.2)