FastQCFastQC Report
Fri 25 Nov 2016
G145-M12_CTTGTA_AC9EP5ANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M12_CTTGTA_AC9EP5ANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19917978
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1142750.5737279155544805Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA298400.14981440385163594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT159150.040.74594511
CGTCGTG161750.040.1858112
TCGTGTA160250.039.79171414
GATCTCG162800.039.50912534
CGTGTAG165700.039.35688415
GATCGGA183250.038.9800761
GTCGTGT167500.038.62189513
TCTCGGT166300.038.44848636
AGCGTCG170150.038.38122610
ATCGGAA186150.038.0890922
GTAGGGA172550.038.07923518
ATCTCGG170050.037.9384335
AGAGCGT176350.037.832848
GTAGATC175500.037.8292331
GTCGCCG157900.037.79397244
GAGCGTC175300.037.3187529
TGGTCGC159750.037.14723242
GTGGTCG164050.037.12436341
GGTCGCC160450.036.95933543
TGTAGGG183500.036.3113217
Produced by FastQC (version 0.11.2)