FastQCFastQC Report
Fri 25 Nov 2016
G145-M11_GGCTAC_AC9EP5ANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG145-M11_GGCTAC_AC9EP5ANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18708667
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC943220.5041620549449087TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA116700.040.32337632
GATCGGA119600.039.3914451
TCGTATG121850.038.67523243
CGTATGC122350.038.35534344
CGGCTAC123900.037.99180633
ACACGTC128100.037.35792513
ATCGGAA126150.037.2039832
CTCGTAT124350.037.04883242
ACGTCTG129450.037.00527615
CGTCTGA129350.036.89785416
AGTCACG130850.036.18005428
TCTCGTA122700.035.57893841
TCACGGC134800.035.0549830
CACACGT138450.034.740212
CACGTCT139200.034.50737414
CACGGCT137850.034.34320431
GTCACGG138000.034.25805329
CGGAAGA140300.033.922034
ATCTCGT130250.033.67655640
GCACACG145600.033.26006711
Produced by FastQC (version 0.11.2)