FastQCFastQC Report
Sat 26 Nov 2016
G122-M5_GATCAG-_BC7TPAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG122-M5_GATCAG-_BC7TPAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16532827
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG383720.2320958176118337Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT63250.040.03257411
TCGTGTA67950.037.23514614
CGTCGTG67900.037.1301512
CGTGTAG68950.036.82275415
AGCGTCG69800.036.37037710
GTCGTGT70450.036.006313
GATCGGA72250.035.1134031
GATCTCG61500.034.51946634
GAGCGTC76250.033.23629
ATCTCGG66050.032.57411635
GTAGGGA78100.032.31198518
GTAGATC69700.031.43775631
TCTCGGT67200.031.42721736
AGAGCGT81350.031.4230698
GTGTAGG82300.031.3848116
GTCGCCG63700.030.77048544
TGTAGGG83100.030.63258217
AAGAGCG84750.030.136397
ATCGGAA85050.029.878962
GTGGTCG68600.029.08585741
Produced by FastQC (version 0.11.2)