FastQCFastQC Report
Fri 25 Nov 2016
G101M12.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M12.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences12402634
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3591702.895917109220509TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA387050.042.5833631
TCGTATG386300.042.42173843
CGTATGC388100.042.20231644
ACACGTC390750.042.1470813
CTCGTAT388650.042.0868442
CGTCTGA391450.042.06030316
ACGTCTG392500.041.95899215
TCACTTA392050.041.7486130
ACTTAGG393750.041.670832
CACACGT395650.041.65290512
CACGTCT395950.041.57117514
CACTTAG396450.041.4413331
ATCGGAA397550.041.4143832
TCTCGTA395600.041.36813441
CTTAGGC398050.041.11660833
ATCTCGT399500.040.9357140
TTAGGCA401000.040.8528734
CGGAAGA407100.040.7250334
TAGGCAT402100.040.68573435
GCACACG409800.040.27907611
Produced by FastQC (version 0.11.2)