FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-TTAGGC_TTAGGC_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-TTAGGC_TTAGGC_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17606617
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT17650.027.78793511
CGTCGTG18150.026.90155612
CGTGTAG19250.026.04910915
AGCGTCG19550.025.31224410
TCGTGTA19750.025.1669214
GATCTCG22500.022.39705534
GATCGGA22450.021.8447781
GTCGCCG22650.021.66456244
GGTCGCC24000.019.9905743
ATCTCGG25350.019.9660235
GTGGTCG24350.019.70334241
GAGCGTC25700.019.511499
AGAGCGT26900.018.6403488
TGGTCGC26750.018.42920742
GTCGTGT28050.018.26939213
ATCGGAA26700.018.1205162
TCTCGGT28450.017.71341336
AAGAGCG30250.016.6486997
GTAGATC32700.016.0154831
CGGTGGT33700.014.95505339
Produced by FastQC (version 0.11.2)