FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-TAGCTT_TAGCTT_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-TAGCTT_TAGCTT_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17612461
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG370100.21013531271978403Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT48250.037.28583511
CGTCGTG49400.036.41805312
AGCGTCG50100.035.9529110
GTCGCCG50050.035.4798544
CGTGTAG51100.034.94706315
TCGTGTA51250.034.8876914
GATCGGA55150.032.9377631
GTGGTCG54000.032.56403441
GGTCGCC54800.032.48951343
GAGCGTC56350.032.121089
GATCTCG56200.032.0694934
GTCGTGT56650.031.71849313
TGGTCGC55850.031.52477542
ATCTCGG57550.031.50866735
TCTCGGT58400.031.1264936
AGAGCGT60450.030.0873228
ATCGGAA61250.029.7295762
AAGAGCG63850.028.4507357
CGGTGGT64650.027.98270439
GTAGATC66150.027.31048431
Produced by FastQC (version 0.11.2)