FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-TAGCTT_TAGCTT_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-TAGCTT_TAGCTT_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17612461
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC364470.2069387123128335TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG49800.036.00314743
CGTATGC50150.035.7087244
GATCGGA52200.035.4002041
CTCGTAT49500.035.1101742
TATCTCG47450.034.4943339
ATCGGAA58250.031.2712612
ACACGTC59200.031.21537613
TCTCGTA53150.030.87781341
CGTCTGA62150.029.62752216
ATCTCGT56850.029.2164540
ACGTCTG64350.028.54624215
CGGAAGA67650.027.9016884
CACACGT66400.027.76431312
CACGTCT66100.027.75719514
TCGGAAG68250.027.5919323
TAGCTTA69700.026.0394734
ACTAGCT71450.025.80196632
GCACACG74650.024.9316811
CACTAGC76700.024.15058931
GTCACTA77500.023.95806529
Produced by FastQC (version 0.11.2)