FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GGCTAC_GGCTAC_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GGCTAC_GGCTAC_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14383790
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT22250.033.21104411
CGTCGTG22450.032.9151812
AGCGTCG24600.030.30664610
TCGTGTA24400.030.01332514
CGTGTAG24600.029.67991615
GTCGCCG23950.029.49320644
GATCGGA26400.028.4052121
GTGGTCG26200.026.96508441
GATCTCG28650.026.19365334
GAGCGTC29550.025.6761789
AGAGCGT29650.025.588878
GTCGTGT28850.025.53714213
ATCTCGG30050.025.19302235
TCTCGGT29850.025.14151436
GGTCGCC28750.024.19004443
ATCGGAA31550.023.6292252
TGGTCGC29350.023.62111942
AAGAGCG33150.022.9535167
CGGTGGT33350.021.77790639
GTAGATC36350.020.52183731
Produced by FastQC (version 0.11.2)