FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GCCAAT_GCCAAT_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GCCAAT_GCCAAT_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23014796
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG29850.029.76739712
GCGTCGT31250.028.1521111
AGCGTCG31950.027.67299710
TCGTGTA31850.027.06851414
CGTGTAG33450.025.97100615
GATCGGA34250.025.6837791
GTCGCCG34450.025.29349944
GATCTCG35550.024.45151334
ATCTCGG36500.024.0564835
GAGCGTC38550.023.2201679
GTGGTCG38600.022.97730341
TGGTCGC40200.022.82923342
ATCGGAA39450.022.0197492
GGTCGCC40250.021.76160643
AGAGCGT41350.021.75338
GTCGTGT42250.021.23915713
TCTCGGT43050.020.70343436
AAGAGCG47700.018.8112747
TCGGAAG49750.017.947143
GTAGATC49600.017.5678431
Produced by FastQC (version 0.11.2)