FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GATCAG_GATCAG_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GATCAG_GATCAG_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17881423
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331330.18529285952242167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG24600.030.67392343
CGTATGC25950.029.33323544
GATCGGA26250.028.7799681
CTCGTAT25750.028.19353142
ACGATCA28350.026.92707332
CGATCAG28850.026.53665733
ATCGGAA30750.024.7536982
ACACGTC33000.023.86592113
AGTCACG34050.022.54848728
GTCACGA34300.022.19191229
TCACGAT34250.022.16007830
TCTCGTA31800.021.58441241
GATCTCG32400.020.98139
ATCTCGT33350.020.3833440
CGTCTGA38050.020.35153216
CACGTCT38650.020.14943514
ACGTCTG38700.019.89601915
CACACGT40450.019.57911712
CACGATC39250.019.56133731
CGGAAGA43800.018.9857084
Produced by FastQC (version 0.11.2)