FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-ATCACG_ATCACG_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-ATCACG_ATCACG_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18664331
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11950.019.6914481
GCGTCGT10200.013.15304711
CGTCGTG10950.012.45310812
TCGTGTA12100.011.26933614
CGTGTAG14200.010.68692515
CGATCAC15100.010.3405393
AGCGTCG13600.09.86478510
GATCGGA14600.09.4896341
CACGATC18350.09.1083411
GTCGCCG15200.08.68583444
GAGCGTC16800.08.6402359
ACGATCA17050.08.5129982
TATGCCG14600.08.2846013
ATCTCGG16600.08.2192435
GATCTCG15550.08.20800434
CGTACGA5101.4859324E-88.19906139
GGTCGCC16400.08.18572943
CGAAGAT20600.07.6864721
GTGGTCG16050.07.67874441
CCACGAT19650.07.4984931
Produced by FastQC (version 0.11.2)