FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-ATCACG_ATCACG_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-ATCACG_ATCACG_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18664331
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT18200.013.4332741
CTCGCAA12950.012.7562131
GATCGGA10800.012.64441
CGTATGC10550.012.30338544
CGAAATA16250.012.0488773
CTCCTAT54200.011.5817951
CTCGGGA35550.011.4000961
CTCCGTT26700.011.05411151
TCGAAAT12850.010.9568772
CTCGCAT11550.010.869841
TCGTATG12350.010.86610643
CTCCGTA14650.010.8249311
CTCGTAT11000.010.79974142
CTCGAAT17700.010.5773281
CTCCGGT20000.010.4622211
GTCGAGT6150.010.3861161
GTCCGTT13150.010.3847541
ATACGTC20600.010.25215331
CTCGTTT24450.09.9994111
TCGGGAT14200.09.91522
Produced by FastQC (version 0.11.2)