FastQCFastQC Report
Wed 23 Nov 2016
WT_control_A_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_control_A_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42872870
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA98550.030.99186541
TCGTATG114800.028.39570243
AAAAGGG171000.026.24211170
CTTGAAA130450.025.41351160
CTCGTAT133700.023.78338842
ATCTCGT130500.023.26893640
CCCGTCC159250.020.77290233
GTCTTCT159050.020.75450752
GCTTGAA158950.020.61584759
CGTATGC186400.017.9548844
TCCATCT187750.016.61788237
GTATGCC199550.016.61487445
CATCTCG189150.016.40261539
TGCTTGA205650.016.22144558
GTCCATC199400.015.82128636
CCGTCTT217150.015.4254150
ACCCGTC219900.015.42316432
AGTCACC224200.015.18906528
CCGTCCA222200.015.02694334
GCCGTCT227200.014.77425249
Produced by FastQC (version 0.11.5)