FastQCFastQC Report
Mon 20 Nov 2017
JG-TeamF-Ec-uvrY-FLAG_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJG-TeamF-Ec-uvrY-FLAG_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17642461
Sequences flagged as poor quality0
Sequence length63-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA38500.028.72677643
TCGTATG48950.024.3703645
CTCGTAT49950.023.32543844
CACGTGA55700.022.41741831
CTTGAAA54350.022.07631162
ATCTCGT51000.021.48131442
TCACGTG60400.020.6154330
GTCTTCT62250.019.66696454
GCTTGAA65450.018.70412861
CGTATGC77050.015.88877946
GAAACGA81200.015.24740736
GTATGCC85000.014.27963547
CCGTCTT87500.014.15059652
TGCTTGA87300.014.10217760
GATCTCG81500.013.86903541
GCCGTCT91900.013.51043951
CTGCTTG98500.012.60477859
ACGATCT92900.012.578155539
AACTCCA249500.012.42472570
ACGTGAA106850.011.91387132
Produced by FastQC (version 0.11.5)