FastQCFastQC Report
Mon 20 Nov 2017
JG-TeamE-Ec-yciT-FLAG_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJG-TeamE-Ec-yciT-FLAG_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13005696
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT358380.27555618707372526TruSeq Adapter, Index 15 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT64800.043.6953542
TCGTATG62850.042.33913845
AGAATCT75200.040.7949639
ACATGTC87450.040.17668532
TCTCGTA70550.039.19727743
GAATCTC76550.038.89475640
CATGTCA93750.037.29128333
CACATGT94750.036.8977131
CGTATGC78100.035.14081246
GTATGCC81500.034.52789347
GCTTGAA71900.034.15452661
CTTGAAA74850.034.016862
GTCTTCT66300.033.73675554
TCACATG105650.033.25557330
AATCTCG89700.032.8067941
GTCACAT111450.031.55629
TGTCAGA112600.031.01638635
GCCGTCT84250.029.97396551
ATGTCAG118650.029.75848634
TCAGAAT118800.029.07291437
Produced by FastQC (version 0.11.5)