FastQCFastQC Report
Mon 20 Nov 2017
JG-TeamE-Ec-aaeR-FLAG_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJG-TeamE-Ec-aaeR-FLAG_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10711766
Sequences flagged as poor quality0
Sequence length61-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT981760.9165248755433978TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA128850.057.20871743
TCGTATG140900.054.414745
CTCGTAT138200.053.6151144
ATCTCGT138600.053.1842942
CTTGAAA146800.052.10584662
AGTCAAC151950.051.48949434
GTCTTCT145750.051.41421554
CACAGTC155550.050.45438831
CGTATGC155150.049.7532246
GCTTGAA154850.049.12683561
AATCTCG151550.048.6626341
GTATGCC160200.048.22759647
CCGTCTT158550.048.0753252
ACAGTCA164550.047.8216532
GCCGTCT160850.047.2967451
TGCTTGA159750.047.2060460
CAGTCAA167050.047.06432333
TCACAGT169100.046.41147230
CAATCTC162950.045.06268740
CTGCTTG166750.044.97510559
Produced by FastQC (version 0.11.5)