FastQCFastQC Report
Sun 14 Apr 2019
SRR6577030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735195
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25820.3511993416712573No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17820.2423846734539816No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13040.17736790919415937No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10120.13765055529485376No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9040.12296057508552152No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8900.12105631839171921No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8090.11003883323472004No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7930.10786253987037453No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7910.10759050319983134No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7850.10677439318820177No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7550.10269384313005393No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7530.10242180645951074No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7470.10160569644788117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3850.067.1647341
GTATCAA15150.054.3081171
TCAACGC18350.044.8100474
ATCAACG18850.043.621453
CAACGCA19050.043.410135
AACGCAG19450.042.7589536
TATCAAC20550.040.0373732
ACGCAGA24500.033.753597
CGCAGAG24950.033.3331348
GGTAATC1900.032.148688
TTAAGAC2100.031.3243543
GTACATG31550.030.8468131
GCAGAGT27250.030.692129
TACATGG31550.029.9527022
ACATGGG33050.028.7179413
TAGGTAT5150.028.2831555
CTTAGGT5150.028.2831553
CTTAAGT1503.704372E-828.191923
CTAAGAC10950.027.8915483
GTCTTAG6250.027.8330671
Produced by FastQC (version 0.11.2)