FastQCFastQC Report
Sun 14 Apr 2019
SRR6577028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568361
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23060.4057280496022774No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16160.28432633484704267No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15340.2698988846877249No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9350.16450812071905005No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8420.14814528090421406No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8120.1428669454800734No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7730.13600510942869057No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7070.12439277149558114No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA6530.114891767732128No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6000.10556670848281285No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC5880.1034553743131566No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA5810.10222376271419044No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG5710.10046431757281024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12500.055.271431
GGTATCA2650.054.9805641
TCAACGC13100.050.2152334
ATCAACG13200.049.8348163
CAACGCA13800.047.6680835
TATCAAC13950.047.1679762
AACGCAG14150.046.4890146
ACGCAGA17800.036.6921847
CGCAGAG18500.035.303838
CGACGGT1750.034.9047137
GCAGAGT19750.033.307329
ATTATGC1755.456968E-1232.2197343
TAGTACT753.2787424E-431.3247434
GACGGTA1807.2759576E-1231.3247418
ACGGTAT1807.2759576E-1231.3247419
ATATACC953.7559974E-529.6760713
TACGACG1901.4551915E-1129.6760715
TACATGG21700.029.6725062
GTACATG22250.028.516561
ACATGGG23600.028.0728073
Produced by FastQC (version 0.11.2)