FastQCFastQC Report
Sun 14 Apr 2019
SRR6577027_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577027_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646653
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24010.37129650678184434No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16370.25314967996746324No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15140.23412865942012173No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8390.12974501007495517No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8350.1291264403010579No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7410.11459005061447175No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7390.1142807657275231No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7190.1111879168580367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3150.055.3682751
GTATCAA13800.054.3110051
ATCAACG14900.049.842083
TCAACGC15000.049.5059784
CAACGCA15550.047.754965
AACGCAG15800.047.594276
TATCAAC17050.042.729982
CTTAGGT5250.038.4976773
ACGCAGA19850.038.1174247
CGCAGAG19900.037.7854928
TTAGGTA5550.036.4139064
GGTATAG5700.036.277397
AGGTATA5650.035.769416
GTCTTAG6200.035.7335361
TAGGTAT5750.035.147335
GCAGAGT22050.034.3143279
GTACATG25800.032.704171
TACATGG25750.032.126312
GTATAGT6850.031.5591628
GGGTTAG600.004100439631.4252381
Produced by FastQC (version 0.11.2)