FastQCFastQC Report
Sun 14 Apr 2019
SRR6577027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646653
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24350.37655434985997127No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17710.2738717673930222No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17530.27108820341048445No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10290.15912707433507614No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9470.14644639397018183No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9190.14211640555290087No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8820.136394635144351No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8020.12402323966640531No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7640.11814682681438114No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6870.10623935866685842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.058.2651181
GTATCAA13300.050.5438961
TCAACGC13900.046.9984554
CAACGCA14550.044.898875
ATCAACG14600.044.7451023
AACGCAG14800.044.140446
TATCAAC16000.041.133192
GTACTAT1401.2732926E-1136.935921
GTCTTAG6350.034.7943271
TACATGG25000.033.2826272
GTACATG24950.032.9724961
ACGCAGA19900.032.5918927
TCTTAGG7100.032.4430772
CGCAGAG20050.032.3480648
TAGGTAT5850.032.1356965
GTCCTAT2500.031.9663621
TTAGGTA5900.031.8633584
CTTAGGT6050.031.8501913
ACATGGG26050.031.5728573
GTATAAG1252.3563734E-730.0859871
Produced by FastQC (version 0.11.2)