FastQCFastQC Report
Sun 14 Apr 2019
SRR6577023_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577023_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699761
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA39920.5704804926253393No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25910.37026927765337025No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23310.33311373454650944No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15090.21564505595481884No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14990.21421599660455498No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11390.16276985999505547No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11230.16048336503463326No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9240.1320450839643821No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8810.12590012875824746No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8460.12089842103232389No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT7980.11403893615105729No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG7960.11375312428100451No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA7720.11032338184037122No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT7530.10760816907486985No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC7410.1058932978545532No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA7230.10332099102407823No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA7220.10317808508905184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.10274936728397266No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA7140.10203483760884073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2900.061.669521
GTATCAA15600.056.114271
TCAACGC18350.046.6088264
ATCAACG18600.045.9823653
CAACGCA18950.044.8850985
AACGCAG19450.043.972856
TATCAAC20400.041.6947362
GTGCAAG4600.035.8092461
ACGCAGA24950.034.2794347
CGCAGAG25550.033.6824348
CTTAGGT7200.033.2866823
GGTAATC3250.033.280348
TTAGGTA7300.032.186964
GTACATG32200.032.0090831
TACATGG32050.031.524142
TAGGTAT7500.031.3286425
GCAGAGT27800.030.9563359
ACATGGG33050.030.1437453
GTCTTAG8800.029.9495531
AGGTATA7900.029.7423826
Produced by FastQC (version 0.11.2)