FastQCFastQC Report
Sun 14 Apr 2019
SRR6577023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699761
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37830.5406131522048242No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26380.3769858565996104No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23050.3293981802358234No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16840.2406535945844367No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15900.22722043669195624No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13290.1899219876500691No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11500.16434182528034572No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9840.14061944006596538No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9460.13518901453496265No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9010.1287582474587752No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8480.12118423290237666No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8400.12004098542216556No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8090.11561090143634754No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT7920.11318150054089897No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG7720.11032338184037122No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA7690.10989466403529206No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC7600.10860851062005457No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG7110.10160611980376158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3600.052.2302861
GTATCAA17850.051.615811
ATCAACG19100.047.233113
TCAACGC19400.046.5026974
CAACGCA20050.044.995135
AACGCAG20200.044.661016
TATCAAC20600.043.581282
ACGCAGA25250.035.7288067
ATAGTAC801.1601818E-535.2403269
CGCAGAG25900.034.8321388
GTACATG31850.034.5359461
TACATGG31950.034.278272
GTGCAAG5250.034.02431
ACATGGG32350.033.1161043
GCAGAGT28250.032.4335759
GGTAATC3850.030.5111058
CGAGATC650.006160828.9151388
AAGGGTA4150.028.3054855
TATATGC1503.6905476E-828.2023372
ACCTAAG12800.027.5433141
Produced by FastQC (version 0.11.2)