FastQCFastQC Report
Sun 14 Apr 2019
SRR6577022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779398
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA40480.51937521009805No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC27800.35668554448433276No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25230.3237113772424358No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18550.2380042032440422No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15760.20220734464291673No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13590.174365343508708No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13190.16923317740101976No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11990.15383667907795504No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT10180.13061362744066574No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9730.12483994056951649No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9450.1212474242941347No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8870.11380578343798675No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT8570.10995665885722057No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8320.10674905503991541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.1062358384291466No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8230.10559431766568557No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA8180.10495279690222455No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG7850.10071875986338173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4300.061.2115971
GTATCAA19700.053.682221
TCAACGC21850.046.4610024
ATCAACG22100.046.1480943
CAACGCA22850.044.6333855
AACGCAG23350.043.2750786
TATCAAC23550.043.1098982
CTAGTAC853.954301E-738.704853
TAGGTAT8550.037.379125
CTTAGGT8850.036.6430933
AGGTATA9150.035.9553266
TTAGGTA9050.035.3139764
ACGCAGA28700.035.2081227
GGTATAG9350.035.186237
GTCTTAG9950.034.4837071
CGCAGAG29450.034.471078
TACATGG35950.033.3392222
GTACATG36050.033.2467421
GTTCGGA1457.4396667E-1032.4129394
GGTAATC3650.032.1909268
Produced by FastQC (version 0.11.2)