FastQCFastQC Report
Sun 14 Apr 2019
SRR6577021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670356
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26900.40127932024178203No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15440.23032537935067338No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15060.22465674954800136No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12270.18303707283890946No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9460.14111904719283486No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7970.11889205138762092No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7800.11635608542326764No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7800.11635608542326764No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7730.11531186414382806No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7430.110836630089087No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7370.10994158327813877No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7350.10964323434115605No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7100.10591387262887182No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7000.10442212794395814No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA6890.10278120879055308No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6780.10114028963714802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14750.058.0142631
GGTATCA2950.052.5953521
TCAACGC16400.050.709954
ATCAACG16700.049.7989963
CAACGCA16950.048.5100945
AACGCAG17550.047.1193546
GTACTAA601.600125E-647.0170561
ATACGGT450.001015193941.7648856
TATCAAC20550.041.4116132
CCTAACG704.620815E-640.300332
ACGCAGA21300.038.6031047
CGCAGAG21750.037.804428
CTTAGGT4750.034.620893
ACCTAAG8700.034.587261
CTAAGAC10150.034.2554363
TAACGCT550.002720318534.171274
CTAACGC550.002720318534.171273
CGCAACG702.1879976E-433.5610665
GCAGAGT24700.033.479549
GTACATG29600.032.880171
Produced by FastQC (version 0.11.2)