FastQCFastQC Report
Sun 14 Apr 2019
SRR6577016_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577016_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818955
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31740.38756708244042715No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20930.25556959784115124No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19070.23285772722554965No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12560.15336618007094407No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11920.14555134286987687No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT9870.12051944246020843No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9170.11197196427154116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16500.055.700711
GGTATCA3550.054.433411
ATCAACG19200.046.5122643
TCAACGC19350.046.39464
CAACGCA19800.044.865415
AACGCAG20500.043.5627026
TAGGTAT6550.040.18485
TATCAAC22500.039.8993572
CTTAGGT6900.038.8276253
TTAGGTA6850.037.7387244
AGGTATA7150.036.8126456
GGTAATC2600.036.1508648
GTCTTAG7900.035.7959751
ACGCAGA25250.035.1772967
GGTATAG7700.034.7893377
CGCAGAG26250.034.1952748
GTACATG31450.034.018511
TACATGG31700.033.3609432
GCAGAGT28000.032.058079
ACATGGG33100.031.6659093
Produced by FastQC (version 0.11.2)