FastQCFastQC Report
Sun 14 Apr 2019
SRR6577013_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577013_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570888
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12020.21054917952382954No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9970.17464020963831786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.15799946749625146No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8690.15221899917321785No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7960.13943190257984053No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7280.12752063452025617No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7230.1266448059864632No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6900.12086433766342962No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6560.11490870363363742No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6230.10912823531060384No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5940.10404842981460462No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5760.10089544709294992No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5720.10019478426591555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14450.051.136751
TCAACGC19050.039.4710924
ATCAACG19300.038.7163123
CAACGCA20000.037.1262635
AACGCAG20050.037.0336766
TATCAAC23550.032.12842
TAGGTAT2800.031.8896485
CTTAGGT2800.031.8896483
CGCACTA300.00415499531.34253518-19
GTACATG28900.031.1054761
GGTATCA5850.030.5723651
TACATGG29650.030.1150132
ACGCAGA24750.029.811147
ACATGGG29450.029.6812253
CGCAGAG25200.029.4678698
ACTCGTT650.006152000328.92278
GTCTTAG3750.028.866751
GGTATAG3100.028.8035537
AAGGGTA1652.9613147E-928.481985
TTAGGTA3250.027.4741574
Produced by FastQC (version 0.11.2)