FastQCFastQC Report
Sun 14 Apr 2019
SRR6577007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614845
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10880.17695516756255641No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10680.17370231521765647No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10640.1730517447486765No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9790.15922712228285177No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9720.1580886239621368No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9350.15207084712407193No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8960.14572778505151707No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8730.1419870048548821No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8640.14052322129967715No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC8580.13954736559620717No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8150.1325537330546723No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7640.12425895957517749No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7180.11677739918190763No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG6990.11368718945425269No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT6970.11336190421976271No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6900.11222340589904774No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6800.11059697972659777No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6640.10799469785067782No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA6590.10718148476445283No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6330.10295277671608291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12050.054.2274481
ATCAACG14050.045.4933663
CAACGCA14650.043.6301545
GTACTAT450.001012513541.7867771
AACGCAG15500.041.540756
TCAACGC15700.040.7122154
GGTATCA3750.040.1153071
TATCAAC19350.034.498392
GGTAATC1256.2591425E-933.8390358
ACGCAGA18900.033.570477
CGCAGAG19400.032.705258
TAGGTAT3400.031.793215
CTTAGGT3300.031.3324383
TTAGGTA3350.030.864794
GTACTAG1551.5133992E-930.3291131
GCAGAGT21450.030.2368989
AGGTATA3600.028.7214016
GTATTAT1503.684181E-828.2060781
AATAGAG1005.3117546E-528.2060782
TACATGG23700.027.571342
Produced by FastQC (version 0.11.2)